Screening of antagonistic Trichoderma for biocontrol activities on phytopathogens

Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XVI
Written by Iuliana RAUT, Mariana CONSTANTIN, Gelu VASILESCU, Luiza JECU, Tatiana ŞESAN

Biological control, the use of specific microorganisms that interfere with plant pathogens and pests, is a nature-friendly and ecological approach to overcome the problems caused by standard chemical methods of plant protection. Trichoderma species are known as biocontrol agents against plant phytopathogens. This finding is a consequence of several key factors, such as faster metabolic rates, anti-microbial metabolites and physiological conformation. In this study, in vitro potential of selected isolates strains of Trichoderma were evaluated against well known and virulent phytopathogens, such as Rhizoctonia solani, Fusarium graminearum, Sclerotinia sclerotiorum, Botrytis allii, Botryris cinerea and Pythium debaryanum. The selection of bioantagonistic microorganisms was carried out using dual culture method observing the pathogen growth inhibition by biocontrol agent. The inhibition extent varies from strain to strain. Trichoderma T27 strain presented the highest bioantagonistic activity and the most sensitive pathogens were Fusarium graminearum, Botryris cinerea and Pythium debaryanum . The optical microscopy observations revealed changes of morphological characters at pathogens due to biocontrol agent activity. Full exploitation of the biocontrol potential of Trichoderma spp. could easily provide growth enhancement of domestic plants, green house plants, and agricultural crops.

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Raut I., Constantin M., Vasilescu G., Jecu L., Şesan T., 2012, Screening of antagonistic Trichoderma for biocontrol activities on phytopathogens. Scientific Bulletin. Series F. Biotechnologies, Vol. XVI, ISSN-L 2285-1364, 63-66.

 


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