The influence of growth regulators concentrations on in vitro micropropagation of Ribes rubrum species

Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XVI
Written by Carmen Gabriela MANOLE, Viorica BĂLAN, Ioana Claudia MENCINICOPSCHI, Diana GOLEA, Steliana RODINO, Alina BUTU

In vitro cultures have found in a short time many practical applications, including genetic improvement of crop species, the multiplication of valuable genotypes, free virus propagation of existent vegetal stocks and conservation of genetic resources. In the present study we aimed to undertake a basic and simple protocol for in vitro micropropagation of Ribes rubrum considering the influence of various concentrations of growth regulators. There were used six variants (M1 ÷ M6) of half-strength MS medium supplemented with growth regulators, as follows: M1 (BA 0.2 mg/l and IBA 0.01 mg/), M2 (BA 0.4 mg/l, IBA 0.02 mg/l and GA3 0.1 mg/l), M3 (BA 0.4 mg/l, IBA 0.02 mg/ and GA3 0.2 mg/l), M4 (BA 0.15 mg/l, IBA 0.015 mg/l and GA3 0.15 mg/l), M5 (BA 0.5 mg/l) and M6 (BA 1 mg/l). The best medium for obtaining micro shoots was half-strength M3 medium. Therefore, the highest rate of in vitro shoot proliferation and elongation was obtained by using combination BA/IBA/GA3 as growth regulators.

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Manole C. G., Bălan V., Mencinicopschi I. C., Golea D., Rodino S., Butu A., 2012, The influence of growth regulators concentrations on in vitro micropropagation of Ribes rubrum species. Scientific Bulletin. Series F. Biotechnologies, Vol. XVI, ISSN-L 2285-1364, 26-29.

 

The semi-solid state cultivation of edible mushrooms on agricultural organic wastes

Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XVI
Written by Marian PETRE, Violeta PETRE

The main aim of this work was focused on testing new practical procedures in order to optimize the efficiency of edible mushroom cultivating by enhancing their fruit body formation during the semi-solid state cultivation on winery and fruit processing wastes. In this respect, the culture media for fungal growing were mainly prepared from winery and apple wastes mixed with relative small amounts of wheat and rye bran (15-30% w/w) as well as germinated barley seeds (3-5% w/w). After their steam sterilization at 121°C, 1.1 atm., for 15 min. they were transferred aseptically inside of 250 ml work volume flasks. These culture media were aseptically inoculated by using 10-20 ml suspension of fungal pellets collected from submerged cultures of the pure strains of Lentinula edodes (Berkeley) Pegler and Pleurotus ostreatus (Jacquin ex Fries) Kummer. After inoculation with mushroom pellets, the semi-solid cultivation was set up for each one of the tested mushroom species following the main parameters of culture media: constant temperature 23°C, pH level 5.7–6.0 units, relative humidity level of culture media 30-50%. During a period of semi-solid cultivation lasting between 30-50 days, the fungal mycelia were developed inside the culture media in the first 10-20 days and after that during the following 20-30 days the first mushroom primordia were emerged and developed the mushroom fruit bodies. In this final stage, the final mushroom fruit bodies were harvested and weighted, all results showing the percentage of 40-50% relative to the whole weight of culture media. Poplar, beech and birch sawdust were used as control samples for the tested culture media.

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Petre M., Petre V., 2012, The semi-solid state cultivation of edible mushrooms on agricultural organic wastes. Scientific Bulletin. Series F. Biotechnologies, Vol. XVI, ISSN-L 2285-1364, 36-39.

 

Variability of polyphenolic composition of quality red wines depending on the origin of raw material and winemaking conditions

Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XVI
Written by Luminiţa VIŞAN, Diana GROPOŞILĂ- CONSTANTINESCU

Our studies refers to the influence of geographical origin of the raw material and the conditions of maceration and fermentation on the composition and extraction of polyphenolic complex at two of the most important varieties of high quality red wines from Romania, Cabernet Sauvignon and Feteasca Neagra; varieties come from different wine regions, Dealu Mare and Murfatlar, harvest of 2010. As regards the oenological practices, our studies aimed three variants of maceration and fermentation of the materials from the mentioned varieties, 5, 10 and 18 day. Analyzes have been carried out in the extracts from epicarp and in the resulted wine by UV-VIS spectrometry techniques. Total content of polyphenols have been determined by IPT technique. Tannins have been determined by the Ribereau-Gayon si Stonestreet method (1996), anthocyanins by dilution technique of the extract with a large amount of solvent (methanol) in strongly acidic conditions and in case of wines was used the discolouration technique with SO2. Color intensity and shade were determined at .=420 nm and .=520 nm. The results of the analyzes have shown significant differences concerning the polyphenolic complex content determined both of the origin of the raw materials as well as the technique used for maceration.

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Vişan L., Gropoşilă- Constantinescu D., 2012, Variability of polyphenolic composition of quality red wines depending on the origin of raw material and winemaking conditions. Scientific Bulletin. Series F. Biotechnologies, Vol. XVI, ISSN-L 2285-1364, 94-96.

 


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