Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XVIII
Written by Roxana STOICA, Silvana Mihaela DANAILA-GUIDEA, Ioana IORDANESCU, Narcisa BABEANU
Starting from the fact that synthesis and accumulation of secondary metabolites can be stimulated by in vitro cellculture from somatic tissues, the purpose of this study was to obtain biologically active substances from callus cellproliferation of varieties of hot chili pepper, like Pintea and De Cayenne genotype (C. annuum L.).Somatic explants taken after 21 days from the regenerated plantlets from germinated seeds in aseptic in vitroconditions, like hypocotyls, cotyledons, young leaves and apex were inoculated on several variants of hormonalcombinations, added to the recipe of basal Murashige and Skoog (1962) culture medium.For the tested variants we used phytohormones, like auxins (NAA and 2.4-D) and cytokinins (kinetin) in concentrationsranging from 0.5 mg/L for kinetin and 0.3-1.0 mg/L for NAA and 2.4 D. The best results on the active growth of callus,were obtained for Pintea variety when there were utilized the cotyledons and apex (100%) and in the case of youngleaves, the result was 58% on media supplemented with kinetin 0.5 mg/L .Comparing with this genotype, for explants of De Cayenne variety cultivated on the same combination of tested culturemedium (MS supplemented with 0.5 mg/L kinetin), the results of 100% were obtained only at the apex level and for theother types of tested somatic explants, the values recorded was 94% in the case of young leaves and only 55% forcotyledons.
[Read full article] [Citation]
callus induction
