ISSN 2285-1364, ISSN CD-ROM 2285-5521, ISSN ONLINE 2285-1372, ISSN-L 2285-1364
 

PHYTOHORMONE–LIKE PRODUCING BACILLUS INCREASE TOMATO SEEDLINGS QUALITY

Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XX
Written by Oana-Alina SICUIA, Sorina DINU, Florica CONSTANTINESCU

Plant growth promoting activity is one of the attributes when searching for beneficial strains of bacteria. Our study aimed to present the potential of Bacillus sp. 83.2s and B. subtilis Bce2 to increase plant growth and vigour. The study is based on phytohormone - like evaluation in the selected Bacillus strains using classic biochemical analysis. Likewise, the plant beneficial effect was also evaluated by growth promotion studies on tomato seedlings, were biometric parameters and chlorophyll content index (CCI) were analyzed. The data have been processed into the following indicators: indole-3-acetic acid in bacterial cultures, and emergence, seedling height and vigour, shoot and root dry weight and CCI in tomatoes seedlings. Both bacterial strains produce high amounts of IAA phytohormones, from 10.4 - 13.8 μg/ml, in normal growth medium, up to 16.3 - 16.6 μg/ml, when 5 mM of tryptophan is added in the medium as auxin precursor. Moreover, B. subtilis Bce2 seed treatment, increased emergence index and seedlings vigour compared to the untreated control and exceeded the commercial growth regulator (Vimpel 77%) in terms of emergence percent, seedlings dry weight, and chlorophyll content index.

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