Optimisation of culture medium to obtain maximal increase of the extracellular starch degradation activity by the enzymes as products released of the Acidiphilium genus

Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XVI
Written by Carmen Mădălina CISMASIU

Acidophilic heterotrophic bacteria belonging to the Acidiphilium genus adapted frequently to the environmental concentration of toxic compounds developing protective mechanisms of resistance which are much diversified in those bacteria. The aim of the present paper was the extracellular enzymes production from the acidophilic heterotrophic bacteria belonging to Acidiphilium populations which hydrolysis the starch in the presence of CaCl2 or/and NaCl solutions. These bacterial populations were isolated from acid waters and mine sediments containing high concentrations of heavy metals collected from Baia (Tulcea County) and Roúia Poieni (Alba County). In the culture medium, the optimum of the substrate concentration represented by starch was 0.2 % in the presence of mixed CaCl2-NaCl solutions. The most important physic-chemical parameter that influence extracellular enzymatic activities of the Acidiphilium genus are the contact time between bacterial populations and culture medium with metallic ions solutions. Also, an extended exposure of Acidiphilium populations in the GYE medium with different starch concentrations and 0.1% CaCl2 induce intense starch degradation by extracellular enzymes in acidic conditions.

[Read full article] [Citation]

Cismasiu C. M., 2012, Optimisation of culture medium to obtain maximal increase of the extracellular starch degradation activity by the enzymes as products released of the Acidiphilium genus. Scientific Bulletin. Series F. Biotechnologies, Vol. XVI, ISSN-L 2285-1364, 103-108.

 


The publisher is not responsible for the opinions published in the Volume. They represent the authors’ point of view.
© 2012 Scientific Bulletin Series F.“Biotechnologies“. All Rights Reserved. To be cited: Scientific Bulletin Series F.“Biotechnologies“.
Powered by INTELTECH DEVELOPMENT.