ISSN 2285-1364, ISSN CD-ROM 2285-5521, ISSN ONLINE 2285-1372, ISSN-L 2285-1364
 

HPTLC PHENOLIC COMPOUNDS FINGERPRINT AND ANTIOXIDANT ACTIVITY OF SAMBUCUS EBULUS LEAVES AND FRUIT

Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XX
Written by Corina BUBUEANU, Ramona-Daniela PĂVĂLOIU, Fawzia SHA'AT, Angela CĂȘĂRICĂ

Phenolic compounds fingerprint of the 50% (v/v) ethanol extracts obtained from leaves (LE) and fruits (FE) of Sambucus ebulus (dwarf elder), fam Adoxaceae, was obtained by high performance thin layer chromatography (HPTLC), in order to evaluated its qualitative chemical composition and antioxidant activity (by DPPH and TAC assays). Quantitative evaluation of total polyphenolic compounds was made by Folin Ciocalteu assay. The obtained fingerprints showed that both extracts are characterized by the presence of flavonoid glycosides and phenol carboxylic acids. The presence of anthocyanins was revealed only in fruit extract Total phenol content was 19.5 mg GAE/g dry material for LE and 52,5 mg GAE/g dry material for FE. Both extracts have important antioxidant activity in a concentration-dependent manner, fruits extract exhibiting a higher one.

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