EVALUATION OF THE GENETIC DIVERSITY OF Pleurotus ostreatus STRAINS USING COMBINATORIAL TUBULIN BASED POLYMORPHISM (cTBP) AND ISSR MARKERS

Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXI
Written by Gabriela POPA, Matilda CIUCĂ, Călina Petruța CORNEA, Bogdan NICOLCIOIU, Radu TOMA

Pleurotus ostreatus is one of the most widely cultivated and popular edible fungi from the genus Pleurotus. In the last years, genetic variation of wild strains and cultivated varieties was investigated by use molecular markers and revealed a reduction of the diversity in commercial cultures. In this paper are presented the results obtained by application of combinatorial tubulin based polymorphism (cTBP) and inter simple sequence repeat (ISSR) markers, in order to investigate the genetic variability in several commercial strains of P. ostreatus collected from different geographical locations and their genetic relationships with wild isolates. Five polymorphic PCR products were obtained with primers designated for intron 1 of beta-tubulin gene, and 12 polymorphic amplicons were detected when primers for intron 2 of the same gene were used. Molecular analysis performed with ISSR primers displayed a polymorphism at intra specific level. The data achieved with the cTBP and ISSR markers revealed a reduced genetic differentiation among the commercial lines dispersed in Europe. However, comparing with commercial lines originated from USA, differences at molecular level were observed, suggesting a possible influence of environmental conditions. Regarding the wild strain of P. ostreatus, the molecular data indicated similarity with all European commercial lines. The results demonstrated that the applied molecular methods could be considered efficient tools to evaluate the genetic diversity in Pleurotus ostreatus.

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POPA G., CIUCĂ M., CORNEA C.P., NICOLCIOIU B., TOMA R. 2017, EVALUATION OF THE GENETIC DIVERSITY OF Pleurotus ostreatus STRAINS USING COMBINATORIAL TUBULIN BASED POLYMORPHISM (cTBP) AND ISSR MARKERS. Scientific Bulletin. Series F. Biotechnologies, Vol. XXI, ISSN 2285-1364, 11-16.


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