TOTAL PHENOLIC ANALYSIS, ANTIMICROBIAL AND ANTIOXIDANT ACTIVITY OF SOME MUSHROOM TINCTURES FROM MEDICINAL AND EDIBLE SPECIES, BY IN VITRO AND IN VIVO TESTS

Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXI
Written by Emanuel VAMANU, Alina VOICA

Wild mushrooms are commonly used in various pathologies. However, there are few studies concerning species characteristics from different geographical areas. The aim of the study was to determine the antioxidant and antimicrobial effects of 17 triple tinctures prepared from 9 species of mushrooms with medicinal potential, harvested from the region of Moldova, Romania. The study was conducted in parallel with seven commercial species by in vitro studies (DPPH and ABTS scavenging and chelating activities) and in vivo (antioxidant activity by using Kluyveromyces marxianus yeast strain). A direct correlation of in vitro antioxidant activity to that expressed in vivo was determined in the case of a high content of phenolic compounds. Tincture prepared from Hericium coralloides presented the lowest antiradical capacity. A correlation between the different in vitro antioxidant activities was determined for the Tuber indicum and Piptoporus betulinus species. Tinctures which have a high degree of protection against oxidative action of H2O2 had a medium value of EC50 < 1 mg/mL. The results obtained proved that certain compounds identified in trace amounts may significantly increase the biological effect, as the protocol for tinctures obtaining directly influenced the response and content expressed in vitro and in vivo.

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VAMANU E., VOICA A. 2017, TOTAL PHENOLIC ANALYSIS, ANTIMICROBIAL AND ANTIOXIDANT ACTIVITY OF SOME MUSHROOM TINCTURES FROM MEDICINAL AND EDIBLE SPECIES, BY IN VITRO AND IN VIVO TESTS. Scientific Bulletin. Series F. Biotechnologies, Vol. XXI, ISSN 2285-1364, 318-324.


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