Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXI
Written by Svetlana COLCERU-MIHUL, Corina BUBUEANU, Ecaterina Anca ŞERBAN, Alice GRIGORE, Domnica RUGHINIS, Cristina BAZDOACĂ, Sultana NIŢĂ

The aim of these studies was to obtain Rosmarini folium selective fractions with beneficial effect in counteracting diseases associated with aging processes. By four different methods 9 selective fractions were obtained. HPLC analysis and quantitative determination of active principles from selective fraction show that the values obtained from individual assessment by HPLC were well correlated with the values obtained by the spectrophotometrically methods. The selective fractions have a total flavonoid content expressed as rutin from 2.004 to 66.970% and respectively 0.651 to 10.284% polyphenolcarboxylic acids expressed as rosmarinic acid. Antioxidant activity evaluation showed that polyphenolcarboxylic acids rich fractions such as RIII: 10.284%, RIIC: 10.257%; had 89.44%; 88.12% antioxidant activity in 1% dilution and 87.52%; 89.07% antioxidant activity in 0.1% dilution, similar to rosmarinic acid who has antioxidant activity of 88.99% respectively 89.84% in dilution of 1% respectively 0.1%. The fractions with 2.642% - 3.950% polyphenolcarboxylic acid content exhibited an antioxidant activity of 45.73% - 87.5% in 1% dilution and of 8.75 to 87.500% activity in 0.1% dilution. Comparing the antioxidant activity of selective fractions and the polyphenolcarboxylic acids content expressed as rosmarinic acid it can be concluded that when the concentration of polyphenolcarboxylic acids increases the antioxidant activity also increases, though not an exact correlation can be made. A correlation between the flavones content of the selective fractions and antioxidant activity can not be made by this method.

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