CANINE AMNIOTIC MEMBRANE DERIVED MESENCHYMAL STEM CELLS- POTENTIAL SOURCES FOR REGENERATIVE MEDICINE

Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXII
Written by Emoke PALL, Raul Alexandru POP, Simona CIUPE, Mihai CENARIU, Ioan Ștefan GROZA

Canine mesenchymal stem cells (MSCs) can be defined with self renew potential and specific differentiation capacity. Amiotic membrane represent an important source of MSCs, which can be harvested by minimally invasive methods. The aim of our study was to evaluate the growth characteristics of canine amniotic membrane derived mesenchymal stem cells. The placenta samples were collected after cesarean section from healthy mixed breed dogs. MSCs isolation was performed using enzymatic method. Isolated cells were cultured in propagation medium: Dulbecco’s Modified Eagle’s Medium/F12 (DMEM/F12, Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% antibioticantimycotic (Sigma-Aldrich). The medium was changed after 4 days. The cell doubling number, cell proliferation capacity, cell doubling time, daily duplication rate and clonogenic efficacy were evaluated. Our study demonstrate the self renew potential of canine amniotic membrane derived mesenchymal stem cells, and can represent a potential source of stem cells for canine regenerative medicine.

[Read full article] [Citation]

PALL E., POP R.A., CIUPE S., CENARIU M., GROZA I.S. 2018, CANINE AMNIOTIC MEMBRANE DERIVED MESENCHYMAL STEM CELLS- POTENTIAL SOURCES FOR REGENERATIVE MEDICINE. Scientific Bulletin. Series F. Biotechnologies, Vol. XXII, ISSN 2285-1364, 211-216.


The publisher is not responsible for the opinions published in the Volume. They represent the authors’ point of view.
© 2012 Scientific Bulletin Series F.“Biotechnologies“. All Rights Reserved. To be cited: Scientific Bulletin Series F.“Biotechnologies“.
Powered by INTELTECH DEVELOPMENT.