Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXIV
Written by Ortansa CSUTAK, Călina Petruţa CORNEA, Viorica CORBU
The yeast Candida (teleomorph Pichia) guilliermondii is a biocontrol agent intensively studied as an ecological alternative for fungicides in preventing decay of plants. The strain C. (P.) guilliermondii CMGB 44, characterized using morpho-physiological tests, was accurately identified using PCR-RFLP of the ITS1-5.8S rRNA-ITS2 region. The phylogenetic genotyping studies confirmed the molecular analysis. The antifungal tests using seven phytopathogenic strains from natural isolates, showed that C. (P.) guilliermondii CMGB 44 had high inhibitory activity against Sclerotium sp., Rhizoctonia solani and Botrytis cinerea. The antimicrobial activity was also tested at 25, respectively, 28oC against four potential pathogenic Candida strains, with relative low results. The activity against Candida krusei and Candida tropicalis was augmented by adding sodium bicarbonate solution in a final concentration of 2%. C. (P.) guilliermondii CMGB 44 presented versatile antimicrobial activity against plant and human pathogenic microbial strains. Future research will aim improvement of the antagonistic activity using an extended range of species/strains.
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