IN VITRO CULTIVATION OF LAETIPORUS SULPHUREUS AND EVALUATION OF ITS ANTIMICROBIAL PROPERTIES

Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XVII
Written by Georgeta FIDLER, Gabriela POPA, Alina BUTU, Steliana RODINO, Calina Petruta CORNEA

Laetiporus sulphureus (Bull. Fr.) Murill., is a wood-rotting basidiomycete mushroom well known for it nutritional value. In this study, alcoholic and aqueous extracts obtained from a Romanian isolate of L. sulphureus cultivated on various culture media were investigated for the antimicrobial operties. PDA, malt extract as solid media and PD (I),malt extract (II), YPG (III) and Hwang (2008)(IV) -as liquid media were used for in vitro cultivation of L. sulphureus,in order to evaluate the optimal medium for an efficient biomass production of L. sulphureus. Between all media tested,best results regarding the growth of mycelia, were obtained when I, II, IV media were used. Only on IV (Hwang)culture medium was observed the typically orange pigment aborated by fungus. Alcoholic and aqueous extracts of fruit bodie and submerged mycelium developed in liquid media were analyzed against strains of Candida albicans ATCC10321, Candida parapsilopsis CBS604, Escherichia coli, Bacillus cereus, Staphylococcus aureus, Enterococcus faecalis, Pseudomonas fluorescens and Pseudomonas aeruginosa. The results shown that the types of culture media for biomass production of L. sulphureus and their aqueous and alcoholic extracts tested against these pathogens have different effects on inhibitory activity. Between the two types of extracts tested aqueous extracts were inferior to alcohol extract in their inhibitory activity on all organisms except Candida sp. in interaction with L. sulphureus aqueous extract from biomass developed on IV medium.

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Fidler G., Popa G., Butu A., Rodino S., Cornea C. P. 2013, IN VITRO CULTIVATION OF LAETIPORUS SULPHUREUS AND EVALUATION OF ITS ANTIMICROBIAL PROPERTIES. Scientific Bulletin. Series F. Biotechnologies, Vol. XVII, ISSN 2285-1364, 11-15.


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