ACCELERATED MICROPROPAGATION OF ENDEMIC FRITILLARIA AUREA SCHOTT

Published in Scientific Bulletin, Series F, Biotechnologies, Vol. XVI, 2012
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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XX
Written by Suleyman KIZIL, Tahsin SOGUT, Ugur SESIZ, Khalid Mahmood KHAWAR

Fritillaria aurea Schott, an Iran-Turan element, is a rare alpine geophyte with spotted brown yellow flowers. The plant is endemic to Turkey with dispersion in the South-Eastern Anatolia region. This study reports in vitro culture of Fritillaria aurea previously collected from the provinces of Adiyaman and Malatya in the South-Eastern Anatolia Region and presently cultured at the Ornamental Plants Garden Collection of Faculty of Agriculture, Dicle University, Diyarbakir, Turkey. Bulblets were cultured on MS medium containing different concentrations of TDZ, TDZ andNAA. Callus induction followed by bulb formation was noted on bulblets. The bulblets obtained these were cultured on MS medium containing 40 or80 g/l sucrose to increase bulb diameter. The in vitro regenerated bulblets were rooted on MS medium containing different concentrations of IBA (0.25, 0.50, 0.75 and 1.0 mg/l) supplemented with 30 g/l sucrose. A significant increase in number of bulblets and bulb diameter was noted on F. aurea Schott bulbs.

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KIZIL S., SOGUT T., SESIZ U., KHAWAR K.M. 2016, ACCELERATED MICROPROPAGATION OF ENDEMIC FRITILLARIA AUREA SCHOTT. Scientific Bulletin. Series F. Biotechnologies, Vol. XX, ISSN 2285-1364, 99-104.

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ADVANTAGES AND DISADVANTAGES OF ACTIVE CARBON IN QuEChERS SAMPLE PREPARATION METHOD FOR PESTICIDE RESIDUES

Published in Scientific Bulletin, Series F, Biotechnologies, Vol. XVI, 2012
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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XX
Written by Vojislava BURSIĆ, Gorica VUKOVIĆ, Tijana ZEREMSKI, Dušan MARINKOVIĆ, Sonja GVOZDENAC, Aleksandra POPOVIĆ, Aleksandra PETROVIĆ

QuEChERS is an analytical method which simplifies the the sample preparation for pesticide residues. It is the result of the necessity to save the time for sample preparation, to reduce the amount of toxic organic solvents and thereby to contribute to the preservation of the environment. In order to obtain a higher recovery, thus making the analysis results more precise and representative, it is necessary during the extraction to pay particular attention to matrix components which can significantly affect the investigation results. In order to decrease the effect of the present components it is necessary to use the adequate substances – sorbents and to examine how their presence affects the validation parameter. The sour cherry extract, as an exceptionally pigmented matrix, needs the use of a sorbent with a strong affinity towards planar molecules thus causing its discoloration by removing the pigments from the extract. The most frequently used sorbents in QuEChERS method of extract purification are primary secondary amine (PSA), graphitized black carbon sorbent (GCB), C18 and Z-Sep sorbent (silicon dioxide coated by zirconium). The paper deals with a possibility of using active carbon (AC) as a possible sorbent which presents a form of carbon obtained in controlled oxidation processes having a porous structure with spacious active surface which enables it to adsorb a wide range of compounds and pollutants from the extract. By use of AC and QuChERS methods an exceptionally purified colorless extract is obtained. The obtained validation parameters point at a very low recovery of the studied pesticides which is a serious disadvantage of this sorbent while high correlation coefficients (R2>0.99) are obtained with irrelevant matrix effect in the linearity investigation process.

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BURSIĆ V., VUKOVIĆ G., ZEREMSKI T., MARINKOVIĆ D., GVOZDENAC S., POPOVIĆ A., PETROVIĆ A. 2016, ADVANTAGES AND DISADVANTAGES OF ACTIVE CARBON IN QuEChERS SAMPLE PREPARATION METHOD FOR PESTICIDE RESIDUES. Scientific Bulletin. Series F. Biotechnologies, Vol. XX, ISSN 2285-1364, 191-194.

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ALGAL OIL: A NOVEL SOURCE OF OMEGA-3 FATTY ACIDS FOR HUMAN NUTRITION

Published in Scientific Bulletin, Series F, Biotechnologies, Vol. XVI, 2012
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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XX
Written by Osman Kadir TOPUZ

Docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) a polyunsaturated fatty acid (PUFA) that belongs to the omega-3 fatty acids group. In recent years, omega-3 fatty acids rich oil has attracted much attention because of its recognized beneficial effect on human health. At present, fish oil is the major source of omega-3 fatty acids, but omega-3 fatty acids rich oils can be produced by microalgae with additional commercial benefits. Consumption of omega-3 rich fish oil has been steadily increasing for decades due to their health benefits. Microalgal oil might be the most promising alternative to fish oil, since they are the primary producers of omega-3 fatty acids. Recent advances regarding production of omega-3 fatty acid rich oil from microalgae have been reviewed with an emphasis on the scientific data. At first, description of the omega-3 fatty acids, biosynthesis pathways and their role in the human health is presented. Microalgae are the initial source of omega-3 fatty acids. Microalgae species intensively used in omega-3 fatty acids rich algal oil production and their culturing conditions were reviewed in this paper. The algal oil extraction and refining process are also presented. We present here a review of the most recent advances regarding the production of omega-3 fatty acid rich algal oil from the marine origin microalgae.

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TOPUZ O.K. 2016, ALGAL OIL: A NOVEL SOURCE OF OMEGA-3 FATTY ACIDS FOR HUMAN NUTRITION. Scientific Bulletin. Series F. Biotechnologies, Vol. XX, ISSN 2285-1364, 178-183.

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AMOUNT OF DNA EXTRACTED FROM DIFFERENT TISSUES OF APPLE TREES IN SPRINGTIME CAN BE USED TO DESCRIBE THE LEVEL OF INFECTION

Published in Scientific Bulletin, Series F, Biotechnologies, Vol. XVI, 2012
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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XX
Written by Desareda MERO, Ariola BACU

Phytoplasma spread in apple trees in a systemic manner or in certain tissues. The unpredictable spreading complicates the definition of a single detection method and requires the use of alternative ones. In this paper are compared the results obtained in three plantations (Turan, Korce and Bitincke), regarding the amount of DNA extract from apples with results obtained from the use of the DAPI staining method for the detection of the infection from the leaves of the same trees. Sampling pool was 10 out of 100 trees which material from roots, stalks and wood were preserved in dark and cold conditions for 24 hours prior to DNA extraction. DNA was isolated from materials mentioned above and leaves were also analyzed by DAPI staining. The data about the amount of extracted DNA from each sample were used to compare the level of infection from different tissues categories and were related to the intensity of staining with DAPI. Results have indicated that the tissues with the highest presence of Phytoplasma spp. in springtime, based on the amount of detected DNA are stalks; and the collection of Korce has the higher level of infection from all analysed collections. At this collection all sampled categories of tissue are infected, meaning that there is a systemic spreading of the disease, compared to the other collections where infection is located only in certain tissues.

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MERO D., BACU A. 2016, AMOUNT OF DNA EXTRACTED FROM DIFFERENT TISSUES OF APPLE TREES IN SPRINGTIME CAN BE USED TO DESCRIBE THE LEVEL OF INFECTION. Scientific Bulletin. Series F. Biotechnologies, Vol. XX, ISSN 2285-1364, 57-60.

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ANTIMICROBIAL ACTIVITY OF TEXTILES TREATED WITH ROSEMARY AND ORANGE ESSENTIAL OILS AGAINST A SELECTION OF PATHOGENIC FUNGI

Published in Scientific Bulletin, Series F, Biotechnologies, Vol. XVI, 2012
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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XX
Written by Ovidiu IORDACHE, Andreea COZEA, Elena VĂRZARU, Elena STOICA, Corneliu PLATON, Steliana RODINO, Iuliana DUMITRESCU

The aim of the research was the evaluation of antimicrobial activity of a textile fabric treated with essential oils extracted from Rosmarinus officinalis (rosemary) and Citrus sinensis (orange), against pathogenic strains of Aspergillus niger (IMI 45551), Candida albicans (ATCC 90028), Trichoderma viride (isolated from agricultural soil), Aspergillus flavus (isolated from agricultural soil) and Epidermophyton floccosum (CCM 8339). Plant extracts were obtained by steam distillation from rosemary vegetal mater and orange peel. GC-MS analysis, carried out in hexane and diethyl-ether, allowed identification of main compounds, with high quantities of eucalyptol, camphor and α –pinene, in rosemary oil, respectively limonene, limonene oxide, α – pinene and β-phellandrene in orange oil. A textile substrate (56% cotton/44% polyester) was treated with concentrations of 1%, 3% and 5% of each oil and antimicrobial activity was assessed against each strain. Bioassays registered various percentage reduction rates, depending on oil concentration and tested strain: on Aspergillus niger, rosemary treatment registered a maximum of 22.12%, whilst orange treatment reached 51.45%; on Candida albicans, both treatment yielded 100% reduction rates for all tested concentrations; on Trichoderma viride, textiles treated with rosemary oil reached a maximum of 76.48% reduction rates, and 100% on orange treatment; on Aspergillus flavus, maximum efficiency on rosemary treatment was of 18.3% and 60.57% on orange treated materials; on Epidermophyton floccosum dermatophyte, maximum reduction rate on rosemary treatment was of 56.99% whilst on orange treatment it registered a maximum of 92.48%. The obtained results promote textiles functionalized with rosemary and orange essential oils as efficient active antimicrobial barriers.

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IORDACHE O., COZEA A., VĂRZARU E., STOICA E., PLATON C., RODINO S., DUMITRESCU I. 2016, ANTIMICROBIAL ACTIVITY OF TEXTILES TREATED WITH ROSEMARY AND ORANGE ESSENTIAL OILS AGAINST A SELECTION OF PATHOGENIC FUNGI. Scientific Bulletin. Series F. Biotechnologies, Vol. XX, ISSN 2285-1364, 362-369.

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BENEFITS OF THE E-LEARNING PLATFORMS AND CLOUD COMPUTING IN THE BIOTECHNOLOGY EDUCATION

Published in Scientific Bulletin, Series F, Biotechnologies, Vol. XVI, 2012
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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XX
Written by Radu Cristian TOMA, Gabriela MARGARIT, Diana GROPOSILA, Dana BARBA

Online education represents a new approach of learning process, in which substantive elements remain the same, only the means of exchange of knowledge and learning is different. Few benefits of the e-learning platforms and cloud computing in educational area are: a reduced infrastructure and IT costs, an increased accessibility, a better collaboration, and allow organizations more flexibility. But cloud computing is having other effects as well, which have the potential to greatly change how education works, both in online and offline (traditional classrooms) courses like: no more expensive textbooks, no more outdated learning materials, no expensive hardware and software required, reaching more diverse students.

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TOMA R.C., MARGARIT G., GROPOSILA D., BARBA D. 2016, BENEFITS OF THE E-LEARNING PLATFORMS AND CLOUD COMPUTING IN THE BIOTECHNOLOGY EDUCATION. Scientific Bulletin. Series F. Biotechnologies, Vol. XX, ISSN 2285-1364, 370-373.

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COLOR CHANGES DURING THE STORAGE IN BOTTLES OF MUSCAT OTTONEL WINE TREATED WITH ASCORBIC ACID AND GLUTATHIONE

Published in Scientific Bulletin, Series F, Biotechnologies, Vol. XVI, 2012
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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XX
Written by Gianina Antonela BADEA, Arina Oana ANTOCE

The present work aimed to determine the colour evolution after aging in bottle of a white wine from Muscat Ottonel cultivar and the effect of the addition of different antioxidants at bottling time, such as SO2, reduced glutathione (GSH) and ascorbic acid. Wine colour is an important indicator of its degree of oxidation, therefore the evolution toward a brownish colour is equivalent with some quality loss or even degradation. Considering that colour is also a key parameter that contributes to the perception of the quality profile of a white wine, the study attempted to determine the effect of certain doses and combinations of some anti-oxidants, which are known to ensure the best wine quality preservation during aging of wine in bottles. Our preliminary study shows that, in some cases and conditions, the antioxidants used to protect the aroma and the colour of wines end up affecting the colour.

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BADEA G.A.,ANTOCE A.O. 2016, COLOR CHANGES DURING THE STORAGE IN BOTTLES OF MUSCAT OTTONEL WINE TREATED WITH ASCORBIC ACID AND GLUTATHIONE. Scientific Bulletin. Series F. Biotechnologies, Vol. XX, ISSN 2285-1364, 121-128.

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COMPARISON OF THREE DETECTION METHODS OF PHYTOPLASMA AT APPLE TREES PROVES THE ADVANTAGE OF AMPLIFICATION OF SPECIFIC 16SrADN

Published in Scientific Bulletin, Series F, Biotechnologies, Vol. XVI, 2012
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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XX
Written by Desareda MERO, Ariola BACU, Margarita HYSKO

During 2015, samples from 30 apple trees growing in three plantations of Korca were tested for phytoplasmic infections. The last have been difficult to detect due to their low concentration especially in woody hosts and their erratic distribution in the sieve tubes of the infected plants. After the evaluation of the sanitary status of three apple collections in Korca district based on classical symptomatology and DAPI staining method, in order to give a final assessment, one 16SrADN sequence of phytoplasma genome was amplified from DNA extracted from roots, stems and trunks. Quality of DNA is of key importance in molecular diagnostics, since it can affect the final result. It depends on sampling material and which plant tissue is examined. Results were used to identify the infected tissues. Results obtained by classical, cytological and molecular methods, were compared to understand the sanitary status of collections and to compare the effectiveness of each of them.

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MERO D., BACU A., HYSKO M. 2016, COMPARISON OF THREE DETECTION METHODS OF PHYTOPLASMA AT APPLE TREES PROVES THE ADVANTAGE OF AMPLIFICATION OF SPECIFIC 16SrADN. Scientific Bulletin. Series F. Biotechnologies, Vol. XX, ISSN 2285-1364, 61-64.

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CULTIVATION OF FLORIDA OYSTER MUSHROOM ON VARIOUS TYPES OF SUBSTRATE

Published in Scientific Bulletin, Series F, Biotechnologies, Vol. XVI, 2012
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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XX
Written by Denisa STĂNESCU, Emanuel VAMANU

Valorisation of agricultural wastes is one of the main objectives for activity optimization in agriculture industry. One method for waste utilization implies their use as substrate for obtaining edible mushrooms, which are a raw material of interest in current food industry. The aim of the paper was the cultivation of Pleurotus ostreatus var. florida species, on diverse common plant wastes. The species had good fructification, resulting numerous pins on the surface of the substrate, the cap diameter reaching a maximum of 4 to 5 cm. The color of the basidium was brighter, because the cultivation temperatures were above 20°C most of the time. The fructification time decreased with the increase of inoculation rate. Although major differences haven’t been determined for morphological characteristics of the fructification body, the supplementation of the substrate formula with other components determined approximately 10% increase in productivity. The study proved that applying supplements to the substrate formula lead to the optimization of valorisation of plant wastes taken into consideration. Also, the use of supplements did not stimulate infection rate of the substrate.

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STĂNESCU D., VAMANU E. 2016, CULTIVATION OF FLORIDA OYSTER MUSHROOM ON VARIOUS TYPES OF SUBSTRATE. Scientific Bulletin. Series F. Biotechnologies, Vol. XX, ISSN 2285-1364, 374-378.

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DETECTION OF GENETIC VARIABILITY IN Pleurotus eryngii USING TUBULIN-BASED POLYMORPHISM MOLECULAR MARKERS

Published in Scientific Bulletin, Series F, Biotechnologies, Vol. XVI, 2012
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Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XX
Written by Gabriela POPA, Catalina VOAIDES, Matilda CIUCA, Calina Petruta CORNEA

Pleurotus eryngii is an edible mushroom with important biological functions, such as anticancer, antiviral, immune potentiating, and hypolipidemic activities. Due to its importance from economically, medicinal and ecologically point of view, a special attention was given to this species worldwide. Analyses via molecular markers revealed that Pleurotuseryngii is a species complex comprises at least six varieties (natural isolates and commercial strains). Improvement of culture technologies as well as the increase of biological compounds content involves a better knowledge of the genetic properties of each isolate, candidate for specific applications. For this reason, in this study several strains of Pleurotus eryngii collected from different geographical locations were analyzed in correlation with a wild Pleurotus ostreatus isolate, in order to determine the genetic variability and relationship between species. The genetic diversity of biological material was analyzed using a TBP (tubulin-based polymorphism) molecular marker that relies on the presence of intron-specific DNA polymorphisms of the plant β-tubulin gene family. For the fungal DNA extraction mycelium obtained on plates with PDA was used. Our results revealed that TBP amplification profile of intron 1 showed eight polymorphic bands while TBP amplification profile of intron 2 indicated nine polymorphic bands.The multiple length polymorphism of β-tubulin intron 2 generated amplification profiles which resulted highly specific for each of the analyzed species. However, these results suggested that TBP molecular markers could be used for highlighting the genetic diversity both intra- and inter-specific and the genetic diversity was closely related to the geographical distribution of this fungus.

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POPA G., VOAIDES C., CIUCA M., CORNEA C.P. 2016, DETECTION OF GENETIC VARIABILITY IN Pleurotus eryngii USING TUBULIN-BASED POLYMORPHISM MOLECULAR MARKERS. Scientific Bulletin. Series F. Biotechnologies, Vol. XX, ISSN 2285-1364, 162-166.

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