Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXII
Written by Maria PETRESCU, Mihaela-Carmen EREMIA
Written by Maria PETRESCU, Mihaela-Carmen EREMIA
This work aims to study the effect of gamma radiation on the inulinase production by the fungus Aspergillus terreus. The fungus was screened for the ability to produce enzymes from other strains belonging to the Collection of Microorganisms of Industrial Importance of the National Institute of Chemical-Pharmaceutical Research and Development. Subsequently, the fungi were irradiated at 3 doses (500, 1000 and 1500 Gy), using the vegetative form of the fungi. A fermentation assay for enzyme production was made using the DNS method. The best enzyme activity was 770.7 U/L, obtained from the media with orange peels. The use of gamma radiation increased the production of enzymes compared to tests without radiation. Statistically, the best dose of radiation was 1000 Gy.
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