ISSN 2285-1364, ISSN CD-ROM 2285-5521, ISSN ONLINE 2285-1372, ISSN-L 2285-1364

Preliminary studies on in vitro behavior of various somatic explants from some cultivated Amaranthus genotypes

Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XVI
Written by Silvana DĂNĂILĂ GUIDEA, Narcisa BĂBEANU, Ovidiu POPA, Denisa STANCIU, Ioana POPA

The recent renewal of interest in underutilized crops of nutritional and economic potential for the agriculture of the future stimulated the research in Amaranthus sp. cultivation and breeding. In vitro systems have important practical applications not only for rapid breeding of this rediscovered crop but also for producing cell biomass to be used as source of phytochemicals of practical interest. The response of explants from hypocotyl, root and cotyledon node of three varieties of Amaranthus species (Amaranthus cruentus“Amont”, Amaranthus hypochondriacus“Intense Purple”and Amaranthus ssp.”Plenitude”) were recorded, upon their cultivation “in vitro” on media supplemented with different combinations of auxins and cytokinins. Our experimental results pointed out that the explant type, the auxin supplement and the genotype were the most important factors in callus initiation. Calluses were induced most frequently on Murashige&Skoog-MS (1962) basal medium with 0.5 mg/l 2,4-dichlorophenoxyacetic acid (2,4­D),whereas the root development occurred in the presence of NAA 1-2 mg/l(a-naphthaleneacetic acid). Proliferative callus derived from cotyledons and hypocotyls of the studied Amaranthus species were transferred to MS media enriched with hydolyzated casein, NAA and kinetin in order to compare their morphogenetic capacity for plant regeneration.

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