ISSN 2285-1364, ISSN CD-ROM 2285-5521, ISSN ONLINE 2285-1372, ISSN-L 2285-1364

Study of high efficiency systems to ractopmine detection in environmental samples

Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XVI
Written by Simone PERAZZOLI, Maíra MALLMANN, Estela NUNES

The concern about the effects of growth promoter drugs exposure, such as ractopamine, has mobilized the scientific community in the search for methods to detect this compound in different matrices. The ractopamine is a E-adrenergic agonist largely used in intensive farm. Studies demonstrates that 95% of the quantity of this ingested drug are excreted in the first 3 days in swine and 55% in cattle are excreted in feces, while 10% and 45% respectively are excreted in the urine, being detected until 2 weeks after the treatment. The present study aimed to propose the development of ractopamine extraction, cleaning and detection methodology to environmental samples (water and wastewater) by a biographical evaluation about it determination in different matrices. For the cleaning and extraction/purification different techniques must be tested, for example the immunoaffinity collumn (IAC) to waters and an association of extraction liquid-liquid (LLE) followed by IAC to wastewaters, because they are complex samples. For the detection, a methodology that uses high performance liquid chromatography with detection by fluorescence (HPLC/FLD) was proposed, because it is less expensive when compared to other high efficiency systems, as liquid chromatography/mass spectrometry (LC/MS) and gas chromatography/mass spectrometry (GC/MS). However, the challenge consists in establishing of a method that achieves the validation rules and it is economically feasible to application not just for research, but in industry and government control departments, mainly in developing countries.

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