Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXI
Written by Ercan YATMAZ, Irfan TURHAN, Mustafa GERMEC, Ercan KARAHALIL

Enzymes are protein-based catalyzers which organize the specific chemical reactions. Therefore, they could be used for lots of industrial applications. For the industrial grade, enzymes were extracted by different physical or chemical methods from the plant or animal tissues formerly. But these techniques are very hard to apply and provide enough enzymes for the industry. The production of the microbial enzymes could be done by a series of operations, which can be divided into two groups (upstream and downstream processes). Production of bulk or partial concentrated enzyme solutions are easier than the production of pure enzyme extracts. In this study, the effects of centrifuge and ultrafiltration on the production of concentrated β-mannanase enzyme extract from fermented carob pod medium by recombinant Aspergillus sojae were investigated. For this purpose, combination of time (5, 10, and 15 min) and speed (7000, 10000, and 15000 rpm) were tested for centrifuge assays. Then, ultrafiltration processes were performed with 10kDa and 30kDa membranes by using 1000 ml fermented media. Because the enzyme molecular weight was 50-60 kDa. Results showed that the centrifuge was not statistically important at β-mannanase enzyme purification in carob medium in point of enzyme activity. But both ultrafiltration membranes were help to improve the specific enzyme activity from 2176.65 U/mg (initial) to 2582.92 U/mg for 10kDa and 2718.89 U/mg for 30kDa (p<0.05). It was obviously seen from the results that 100ml concentrated enzyme extract was collected from the retentate.

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