Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XIX
Written by Nihad. H.M. Al- Ezerjawi
This study was conducted to evaluate the effect of biocontrol fungi - Trichoderma harzianum.austaralian(T.h.a). and Trichoderma harzianum.Rafai (T.h.n) and Chaetomium elatum (C.e) isolates in improvement of the growth of rice seedlings - class anber -33 and combating Echinochloa weeds which accompaniment to rice cultivation.To attain these aims , three laboratorial experements were carryied out. The first experement included isolation, purification and estimation of fungi frequency in rice field (AL-Najaf AL-Ashraf Governorate), while the second experiment included the use of fungi and their filtrates by planting rice and Echinochloa seeds in petri-dish containing biocontrol fungi. The third experiment acheived by using a spots that containing a soils (200 gm of field samples) treated with studied fungi (held on powerded wheat straw) and comparing the germination and growth ( percentage of germination , lengths (cm), fresh and dry weights (gm) of plumile and radicle of rice and Echinochloa seedlings).These experiments was designed by completely randomized design(C.R.D) Results of this study can be summarized as follows. ………….……………… 1- The genous Aspergillus showed the frequency of 23.53% at the start of growing, while Trichoderma reached at the highest frequency of 39.00% at the end of growing. 2- T.h.t. and Rhizoctonia solani (non pathogenic isolate ) and their filtrates showed an important results in the field of controlling of Echinochloa weeds grown in petri dishes and spots , Echinochloa germination percentage reached 8.22, 16.55% in petri dishes and 20.56, 12.65% in spots respectively as well as fungi filterates achieved 12.50, 17.50% in petri dishes respectively, in addition T.h.t. and R. solani attained a asignificant differences on rice seedlings growth parameters germination percentage reached 95.32, 80.16% in petri dishes and 93.50,77.50% in spots respectively, as well as fungi filterates acheived 93.51, 81.50% in petri dishes respectively. Also T.h.t. and R. solani and its filtrates attained asignificant results in combating with Echinochloa germination percentage and in reducing of plumile and radicle length ,fresh and dry weight in compare with control treatment.
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biological control
