Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XIX
Written by Silvana-Mihaela DĂNĂILĂ-GUIDEA, Ricuţa-Vasilica DOBRINOIU, Luminiţa VIŞAN, Radu Cristian TOMA
The plant known scientifically as Lycium barbarum L., and commonly in the West as the wolfberry, or simply as "goji", is considered by many authors as the most nutrient rich plant on earth. It has been used for thousands of years by Chinese and Tibetan therapists as a source of health. The plant Lycium (Goji) is a shrub of the family Solanaceae is a true national treasure for China used in traditional medicine for over 2000 years.Thus, according to information published in various specialized articles and those taken from traditional beliefs, goji fruit is considered to be an important antioxidant, antidiabetic, and a natural source with excellent effects on the cardiovascular system and in decreasing the level of cholesterol in the human body. Given the properties of this imported super fruit the initiative to develop a propagation protocol to be widely used in Romania too is therefore considered to be one relevant for this plant species. The direct organogenesis protocol used in this study yields explants and microcuttings from meristematic apexes consisting of 2-3 node fragments detached from 30 day old goji seedlings germinated in in vitro conditions. The average was 89-95% for morphogenetic culture; for the offshoots rooted in a liquid culture supplemented with 1 mg / l IBA, it was 100% .In vitro goji rooted plantlets were successfully acclimatized (average survival rate of 90-98%) in a peat substrate mixed with sand. After being placed under ex vitro conditions, the vitroplantlets increased rapidly and developed well. Over a period of one month, they formed new branched roots and many axillary shoots with healthy leaves.
[Read full article] [Citation]
direct organogenesis
