Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XVII
Written by Andreea NISTOR, Mihaela CIOLOCA, Nicoleta CHIRU, Monica POPA
The callus is an unorganized mass of parenchymal proliferate cells that through cultivation, forming groups of meristematic cells, elements of leading system, pigmented cells, etc.. Using other explants than meristem, for regeneration neoplantlets require mandatory completion of a stage of callus culture. To obtain callus is need an agarose to support the cellular mass in growth. In 2012, at Brasov was fitted trifactorial experience,in which two Clones of Christian variety were studied, 6 media for callus induction and 2 explants sources consisting of leaf disc and petiole segment. The following results were obtained: medium, explant source (foliar disc, petiole segment) and variety have different influences on callus proliferation. The callus explants responded better to the foliar disk (72.5%) than petiole segments (40%). Media containing 3 mg / l 2,4-D and 3 mg / l BAP x 3 mg / l 2,4-D favored callus induction rate of 90%. Differences obtained by using BAP citokinine are statistically assured, very significant, negative compared to 2,4-D auxine (2mg/l- concentration regarded as control), by -3.5 explants / that induced callus. The callus which was obtained will be used for plantlets regeneration and to identify any somaclonal variation.
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callus
