ISSN 2285-1364, ISSN CD-ROM 2285-5521, ISSN ONLINE 2285-1372, ISSN-L 2285-1364


Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XVII
Written by Mariana CĂLIN, Iuliana RAUŢ, Olguta DRACEA, Luiza JECU, Veronica LAZĂR

Dermatophytosis has become one of the most common human infectious diseases in the world so it is of interest to dedicate more studies to its etiological agent’s dermatophytes. These keratinophilic and keratinolytic filamentous fungi have the ability to invade and colonize keratinized layers of the skin and their appendages. Dermatophytes fungi group include three anamorphic genera namely Epidermophyton, Microsporum and Trichophyton. These three genera include geophilic, zoophilic and anthropophilic species. Usually these filamentous fungi are identified on the basis of conidia morphology and sometimes with specific physiological characters, such as the hair strand perforation and urea hydrolysis. The objective of present study was to isolate and to identify some filamentous dermatophytes fungi from human superficial mycoses. Isolated samples (scales, fragments of nails and subungual debris) were cultured on specific culture media during four months. After incubation time, morphological characters of cultured fungal were observed macroscopically and microscopically. The following cultural characteristics were analyzed: texture, surface and reverse color of the colony, the presence of pigmentation. Microscopic examination offered data on specific characters such as, the presence/absence of macroconidia and microconidia, theirs shape, their septa number, the presence of chlamydoconidia, spiral hyphae and nodular organs. The strains identification was completed with in vitro hair strand perforation and urea hydrolysis. The isolated microbial strains were identified as belonging to Trichophyton and Microsporum genera.

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