Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXIV
Written by Doina CLAPA, Orsolya BORSAI, Loredana LEOPOLD, Cristina COMAN, Alexandra TOMA, Ioana OPREA, Monica Hârța
The aim of this study was to investigate the effects of two different nanoparticles TiO2 (TiO2 NPs) and ZnO2 (ZnO2 NPs) on the in vitro culture of soybean plants (cv. Felix.). The TiO2 and ZnO2 NPs concentrations used for soybean tissue culture were 10, 100 and 1000 mg/l added to MS medium which contained no plant growth regulators. After four weeks, average height of the plants, average length of the main roots, secondary roots and fresh weight of the plantlets were measured. The results show that relatively low concentrations of TiO2 NPs (10 and 100 mg/l) when added to the culture medium didn’t have deleterious effects, but stimulated the growth and development of soybean plants. When higher concentrations (1000 mg/l) of TiO2 NPs were added the plant growth was inhibited. The average fresh weight of a plantlet was 2200,14±51,56 mg on the medium without TiO2 NPs and 2270,03±39,78 mg at the concentration of 100 mg/l TiO2 NPs added. At the highest concentration of TiO2 NPs added the average weight of the plant decreased significantly to 1616,86 ± 68,09 mg. The addition of ZnO2 NPs to the culture media at higher concentrations than 10 mg/l showed a clear inhibitory effect on plant growth. Therefore, our results suggest that ZnO2 NPs had a greater inhibitory effect on soybean plant growth and development than the TiO2 NPs.
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