ISSN 2285-1364, ISSN CD-ROM 2285-5521, ISSN ONLINE 2285-1372, ISSN-L 2285-1364


Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XVIII
Written by Mihail MAGHER, Natalia LEMANOVA, Maria MAGHER

Crown gall is a widespread disease of cultivated culture all over the world. The bacterial pathogen Agrobacterium tumefacience colonize the xylem vessels of the plants.The tumors form in stems and roots of fruits culture. Large tumoursmay death of new plants in the nursery and in the plantation. Tumouregenesis causing root decay may be factor involved in the complex syndrome. Development of measures to controle crown-gall is carried out in direction for obtaining tumorless plants at the expens of preventive treatment of woundings so as to deteriorate the interaction of pathogen with the cell of host plant. Utilization the strain of soil inhibiting Pseudomonas fluorescens CR- 330 D which synthesize the substances (bacteriocins) with preventing activity to nopaline and octopine strains of pathogenic agrobacteria. Liquid concentrate suspension of this bacteria - biological preparat < Paurin> -were applicated for preplanting treatment of rooted saplings. Using to treat apple MM-106 wilding prior to planting into fruit nursery allowed to decreases the outcome of apple saplings affected by crown gall down to 2 – 2.5 %. He is capable to compete with soil microflora and get reliably fixed in rhisosphere without phytotoxicity, which is rather advantageous for using biological control against repeated inoculation with crown gall pathogen.

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