Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXV, Issue 2
Written by Agnes TOMA (APATEAN), Lucia MOLDOVAN, Oana CRACIUNESCU, Stefana JURCOANE, Florentina ISRAEL-ROMING, Daniela BALAN, Gabriela LUTA
Collagen is a natural biopolymer, widely used in various fields, including medical, pharmaceutical, cosmetic or food industry. It is obtained from terrestrial and aquatic sources. Waste from fish meat processing industry is an important source of collagen. The purpose of this paper was to show the possibility to obtain high quality collagen from the residues of the fish processing industry, the main methods of getting and characterizing it,as well as the possibilities of using it. The main methods of collagen obtaining are chemical methods using acetic acid, sodium chloride and sodium hydroxide and enzymatic methods using pepsin, trypsin or pronase. A more recent method, such as ultrasound improves the yield of collagen and its quality. In the case of hard tissues (bones, scales) it is necessary a decalcification often using EDTA or hydrochloric acid. The soft (meat, skin) and hard tissues are mainly treated with sodium hydroxide, butyl alcohol, acetone, etc. to remove non-collagenous proteins and fats. The obtained collagen is analyzed in terms of yield, hydroxyproline content, amino acids analysis, infrared spectra, molecular weight and denaturation temperature determination.
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