Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXVI, Issue 1
Written by Nazar AL GHASHEEM, Florin STĂNICĂ, Adrian George PETICILĂ, Oana VENAT, Ana BUTCARU, Imad Al-SUWAID, Basim K. HASAN, Ihasn J. AZEB, Kawther ALGHASHEEM
Three peach varieties (Florin, Filip and Mimi), two explants (shoots-tip and nodes) were tested. The explants were cultivated on 3 medium MS, B5 and QL adding of 30 g/l sucrose and 7 g/l agar without any hormone supplements during initiation stage. Cultures tubes were kept in the dark (0, 1, 2, 3, 4) days to get rid of the process of oxidation of phenolic materials resulting from cutting plant tissues. It was found during this study that the placement of the tubes of culture in the dark has a positive effect in the disposal of phenolic materials. Results presented that placing the explants in dark for 2-3 days was the best result to explants phenolic-free, in 2 days/dark (Florin 89% explant growing, Filip 83% explant growing and Mimi 85% explant growing), in 3 days/dark (Florin 89% explant growing, Filip 84% explant growing and Mimi 86% explant growing). Also QL medium give yellowish green shoots with a high resistance 95% to vitrification.
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