Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXVI, Issue 2
Written by Maria Monica PETRESCU, Dana Maria MIU, Gabriela SAVOIU, Mihaela Carmen EREMIA
Inulinases are an important class of enzymes used in many fields, especially in the food and pharmaceutical industries, to produce fructose syrups. Microbial inulinases are important in the hydrolysis of inulin to produce fructose syrup and FOS. These enzymes are produced by various strains of microorganisms, of which Aspergillus sp. and Kluyveromyces sp. are the most commonly used strains for inulinase production. The goal of the study was to biosynthesis inulinase using the Aspergillus terreus ICCF 262 strain, with inulin and orange peel powder as carbon and energy sources, the enzyme being isolated from the fermentation medium by fractional precipitation with ammonium sulfate followed by purification on DEAE-Cellulose using ion exchange chromatography. Within 7 days of cultivating the fungal strain on a mineral medium containing inulin and orange peel at a final concentration of 2% in the fermentation medium, yields of biotechnological interest were higher than those previously reported in the literature. Through the procedure of isolating and purifying inulinase from the fermentation medium results a specific activity of between 164.6 - 396.4 U / mg protein.
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