Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXVII, Issue 1
Written by Ionuț GIURESCU, Tatiana-Eugenia ȘESAN, Stere BADJU, Carmen LUPU, Florin OANCEA
This paper presents a process for preparing from sea buckthorn branches sawdust of a compost by using a multipurpose Trichoderma strain (T. harzianum Td50b). The used strain accelerates the degradation of the lignocellulosic material and has biocontrol and plant biostimulant characteristics. The branches, resulting as a by-product of harvesting sea buckthorn berries and leaves, were grounded and extracted to recover bioactive ingredients - flavonols, flavones, phenolic acids, proanthocyanidins, triterpenoids, hydrolysable tannins, serotonin. The resulting sawdust, depleted in polyphenols with potential anti-fungal activity, was normalized to 90% water activity with ammonium nitrate solution 0.2M, supplemented with 10% eggshells powder, and inoculated with 2% alginates beads granules containing 109spores T. harzianum Td50b per gram. After incubation for 50 days at room temperature in aerobic conditions, the survival of Trichoderma was analyzed by cultivation on selective media. The Trichoderma re-isolated from sea buckthorn branch compost maintained its antagonistic and plant biostimulant characteristics.
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