Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXVII, Issue 1
Written by Asawir Esamaldeen Ebrahim MOHAMED, Oana-Alina BOIU-SICUIA, Călina Petruța CORNEA
Mycotoxigenic contaminants in feed raw materials are a serious problem in animal production due to their negative impact on animal performance and health. The most hazardous genera of mycotoxigenic fungi that can contaminate feed raw materials are Aspergillus, Fusarium, and Penicillium. However, not all fungi of these genera are mycotoxin producers. Therefore, to differentiate contaminated from healthy feed it is important to apply fast detection methods. This review paper aims to present fast qualitative detection methods of mycotoxigenic contaminants. Studies are focused on molecular techniques such as PCR (polymerase chain reaction), as well as chromatographic method such as TLC (thin layer chromatography). Using the PCR method, there can be detected either the fungal species and genera, either the presence of certain genes or gene clusters encoding for mycotoxin synthesis. Mycotoxin detection through TLC is an affinity-based method, in which non-volatile compounds are detected based on their retention factor (Rf) depending on the stationary and mobile phase used. The mycotoxigenic contaminants reviewed in this paper are Aspergillus, Fusarium and Penicillium and their producers.
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