Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XIX
Written by Lobo Balia ROOSTITA, Khusnul KHOTIMAH, HUNAINAH, Ratu SAFITRI, Mia MIRANTI, Hartati CHAIRUNNISA, Gemilang Lara UTAMA
Aims of the research was to characterize probiotic properties of Enterococcus bacteria isolated from bovine colostrum. The research done experimentally with factorial pattern of Completely Randomized Design including two factors such type of bacteria and the characters of probiotics and replicated three times. Parameters of the research was pH tolerance, bile salts, antimicrobial activity, the ability of auto-aggregation. Data analyzed by ANOVA and Duncan’s Multiple Range Test (DMRT). Two Enterococcus bacteria isolated from the colostrum which identified with the API 20 Strep were known as E. faecalis and E. faecium. The result showed that E. faecalis and E. faecium were pH 4 tolerant with the population of 1.623 x 102 and 1.316 x 102 CFU/ml. Availability to survive at high bile salt concentrations (0.3% and 0.5%) which shown by 15.683 x 1010 and 23.667 x 1010 CFU/ml population. Enterococcus faecalis and E. faecium also had antimicrobial properties which indicated by clear zone diameter towards E. coli (9.7 and 10.3 mm), S. thypimurium (9.7 and 10 mm), and L. monocytogenes (9 and 12 mm). The inherent ability of the isolated Enterococcus was better than the test bacteria (E. coli, S. thypimurium, Listeria) with the auto-aggregation values were 61.783% and 60.425%.
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