Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XIX
Written by Sorin Septimiu COMAN
The goal of achieving a high quality final product of Collagen type 1 suitable for both cosmetical and pharmaceutical industry is a constant battle between the production cost and maintaining a superior improved standard working protocol. The aim of this study is to optimize the production of Collagen type 1 from different waste materials such as eggshells and methanol by using a fermentation protocol with genetic modified Pichia pastoris yeast. In our first experiment we used as carbon sources a mixture of eggshells and a glycerol and methanol feeding solution and a fermentation procedure with oxygen limited strategy. The intermediary product was lyophilized and then capped in sterile bottles. The production method that we tried to optimize regarding the profitability and also the obtaining of high quality final product of Collagen type 1 was successfully achieved. Taking into considerations this result, we believe that the productions costs could be further lowered by adding as a carbon source from marine Black sea algae such as Ulva familae, Porphyra leucostica or Punctaria latifolia.
[Read full article] [Citation]
Collagen type 1
