Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXVIII, Issue 2
Written by Andrei Constantin Ioan ARLET, Brindusa BALANUCA, Mihaela TOCIU, Cristina OTT, Lucian CONSTANTIN, Aglaia POPA, Florentina ISRAEL-ROMING
Different extracts from coloured corn varieties are well known for their profile of high-value phenolic compounds. In this context, the current study proposes an evaluation of the phenolic content for the hydroalcoholic extract obtained from bran of red corn, a variety grown in Romania. Therefore, the native extract, obtained by ultrasounds treatment, was separated using different membranes (regenerated cellulose and polyethersulphone), with nominal molecular weight cut-off of 5000 and 10000 KDa, by employing different working parameters. Several extract fractions were obtained and characterized in terms of their total phenolic content and total flavonoid content by means of UV-Vis spectrophotometry and phenolic acids content also, by HPLC. Total antioxidant capacity was also measured by means of UV-Vis spectrophotometry. Membrane separation has been observed to lead to extract fractions rich in phenolic compounds, especially with flavonic structures, such as rutin and quercetin, with great potential for today's modern industries (pharmaceutical, cosmetic or food industry).
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