Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XX
Written by Gabriela POPA, Catalina VOAIDES, Matilda CIUCA, Calina Petruta CORNEA
Pleurotus eryngii is an edible mushroom with important biological functions, such as anticancer, antiviral, immune potentiating, and hypolipidemic activities. Due to its importance from economically, medicinal and ecologically point of view, a special attention was given to this species worldwide. Analyses via molecular markers revealed that Pleurotuseryngii is a species complex comprises at least six varieties (natural isolates and commercial strains). Improvement of culture technologies as well as the increase of biological compounds content involves a better knowledge of the genetic properties of each isolate, candidate for specific applications. For this reason, in this study several strains of Pleurotus eryngii collected from different geographical locations were analyzed in correlation with a wild Pleurotus ostreatus isolate, in order to determine the genetic variability and relationship between species. The genetic diversity of biological material was analyzed using a TBP (tubulin-based polymorphism) molecular marker that relies on the presence of intron-specific DNA polymorphisms of the plant β-tubulin gene family. For the fungal DNA extraction mycelium obtained on plates with PDA was used. Our results revealed that TBP amplification profile of intron 1 showed eight polymorphic bands while TBP amplification profile of intron 2 indicated nine polymorphic bands.The multiple length polymorphism of β-tubulin intron 2 generated amplification profiles which resulted highly specific for each of the analyzed species. However, these results suggested that TBP molecular markers could be used for highlighting the genetic diversity both intra- and inter-specific and the genetic diversity was closely related to the geographical distribution of this fungus.
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