Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXII
Written by Diana GROPOŞILĂ-CONSTANTINESCU, Luminiţa VIŞAN, Radu-Cristian TOMA, Gabriela MĂRGĂRIT, Dana BARBA

The theme of the paper was chosen because we considered it important that people know not only the negative part about whey - it is a waste, but also the positive side - that it can be successfully used in the production of value-added products. The purpose of the paper was to obtain a probiotic product by superior capitalizing of whey. The objectives of the paper were: (1) Processing of whey in a slightly fermentable form for the cultivation of Saccharomyces cerevisiae and Lactobacillus bulgaricus strains; (2) Optimizing the composition of the whey culture medium in order to obtain the highest biomass productivity; (3) Obtaining probiotics based on Saccharomyces cerevisiae and Lactobacillus bulgaricus strains. Several media variants with different glucose concentrations (0%, 0.5%, 1%, 2%) were used for the biosynthesis of probiotics. In the case of yeasts, glucose supplementation of culture media increases the amount of wet biomass by 21% compared to glucose-free media, and 16% for lactobacilli. However, good results were also obtained on whey as such, without additional glucose, yielding 52 g/l yeast and 42 g/l lactobacilli.

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