Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XX
Written by Mihaela DUȚU, Aurel ARDELEAN, Mirela ARDELEAN, Dorina CACHIŢĂ-COSMA, BURDUCEA Marian, Andrei LOBIUC, Elida ROSENHECH
According to information published in various specialized articles and those taken from traditional beliefs, goji fruit is considered to be an important antioxidant, antidiabetic, and a natural source with excellent effects on the cardiovascular system and in decreasing the level of cholesterol in the human body. The present study was conducted to develop a method of optimizing the content of antioxidants. Therefore, we initiated the in vitro culture of goji plants from meristematic apexes of plants harvested from 30-day-old germinated seeds generated from the substrate septic consisting of peat mixed with perlite. The culture medium used was Murashige & Skoog supplemented with 0.5 mg / l IBA (indolyl butyric acid) and 0.5 mg / l BA (benzyladenine). Plants containers were exposed to light of different colors fluorescent tubes from Osram company and the following lengths wave: white; blue- 473 nm; green- 533 nm; yellow 580 nm; red-680 nm. Chamber growth temperature was 23°C ± 2°C, between light and 20°C ± 2°C, during hours of darkness, photoperiod of 16 hours light and 8 hours darkness. At the age of 60 days these vitro seedlings have undergone physiological and biochemical analyzes. Red light, green and yellow stimulates growth length of vitro seedlings and blue light produce small and stocky plants and even growth slowing. Blue light has increased the total content of assimilating pigments and content of antioxidants (phenols, flavonoids) were increased by the yellow and white light. The antioxidant capacity was also higher in vitroplants grown in blue light and lowest in red light.
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