Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XX
Written by Desareda MERO, Ariola BACU
Phytoplasma spread in apple trees in a systemic manner or in certain tissues. The unpredictable spreading complicates the definition of a single detection method and requires the use of alternative ones. In this paper are compared the results obtained in three plantations (Turan, Korce and Bitincke), regarding the amount of DNA extract from apples with results obtained from the use of the DAPI staining method for the detection of the infection from the leaves of the same trees. Sampling pool was 10 out of 100 trees which material from roots, stalks and wood were preserved in dark and cold conditions for 24 hours prior to DNA extraction. DNA was isolated from materials mentioned above and leaves were also analyzed by DAPI staining. The data about the amount of extracted DNA from each sample were used to compare the level of infection from different tissues categories and were related to the intensity of staining with DAPI. Results have indicated that the tissues with the highest presence of Phytoplasma spp. in springtime, based on the amount of detected DNA are stalks; and the collection of Korce has the higher level of infection from all analysed collections. At this collection all sampled categories of tissue are infected, meaning that there is a systemic spreading of the disease, compared to the other collections where infection is located only in certain tissues.
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