Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XX
Written by Desareda MERO, Ariola BACU, Margarita HYSKO
During 2015, samples from 30 apple trees growing in three plantations of Korca were tested for phytoplasmic infections. The last have been difficult to detect due to their low concentration especially in woody hosts and their erratic distribution in the sieve tubes of the infected plants. After the evaluation of the sanitary status of three apple collections in Korca district based on classical symptomatology and DAPI staining method, in order to give a final assessment, one 16SrADN sequence of phytoplasma genome was amplified from DNA extracted from roots, stems and trunks. Quality of DNA is of key importance in molecular diagnostics, since it can affect the final result. It depends on sampling material and which plant tissue is examined. Results were used to identify the infected tissues. Results obtained by classical, cytological and molecular methods, were compared to understand the sanitary status of collections and to compare the effectiveness of each of them.
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