Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XX
Written by Tetyana KYRPA-NESMIIAN
An important role in plant resistance to low temperature plays a composition of membrane lipids. With the increase of unsaturated fatty acids (FA) in the membranes of cells decreases the transition temperature of the gel phase in liquid crystal phase. Desaturases are enzymes that contribute to the formation of double bonds in the crystal and thus turn the FA with a saturated in unsaturated. The paper used the plant Nicotiana tabacum, expressing the gene of Δ9 acyl-lipid desaturase (desC) cyanobacterium Synechococcus vulcanus. These plants were transformed with a vector-based pBISN with selective nptII gene, under the control of the 35S cauliflower mosaic virus, that carrying the targeted gene desA: licBM3 by Agrobacterium-mediated transformation. Spend regeneration on Murashige-Skooge medium with the addition of ВАР, NAA, cefotaxime, kanamycin. Finally we obtained transgenic plants carrying the two genes heterologous desaturases cyanobacterium.
[Read full article] [Citation]
