Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XX
Written by Alina SIMINA, Manuela CRISAN, Sorin CIULCA, Dorica BOTAU
Medicinal plants and their products are an important solution to improve the treatment of people in the whole world. Momordica charantia L. is a well-known species for its biological activity (antioxidant and antimicrobial activity) and contains a complex of beneficial compounds such as: vitamins, minerals and antioxidants that can be used for treating a wide range of illnesses, especially diabetes. Plant tissue culture is an important and facile method for the somatic variability induction and tissue lines selection inorder to obtain valuable secondary metabolites. The use of the substances that control growth and synthesis capacity of tissues allow us to produce under aseptic conditions significant quantities of plant metabolites. The controlled conditions give to the tissue culture a suitable microenvironment for the successful growth and biosynthesis. Phytohormons and other substances with the same effect can determine in tissue culture the increase of biosynthetic capacity which can lead to obtaining and selection of proliferative tissue lines producing secondary metabolites. The present work aims to study the influence on M. charantia L. tissue culture of a new biological active compound, diethanolamine salt of 4-nitrobenzoic acid (4-NO2BA DEA), synthesized by the Institute of Chemistry Timisoara of Romanian Academy. In this research we used 6 hormonal balances in which we associated 4-NO2BA DEA with cytokinin BAP and also the new compound alone on the MS culture medium, for the selection of tissue lines with high growth capacity.We note that increasing the amount of 4-NO2BA DEA in the MS culture medium could have beneficial effects on tissue culture at M. charantia.
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