Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XX
Written by Andreea DOBRE, Laura Alexandra MARIN, Carmen MANOLE, Nicoleta ANDREI, Călina Petruța CORNEA
Microorganisms are the main responsible for bioremediation processes. They have the capacity to convert toxic substances in lesser or untoxic ones and also can solubilize compounds in order to make them available to plants. This article emphasizes the capacity of several microorganisms, isolated from different sources, to solubilize Pin, Porg and Zn based compounds. The screening methods comprised the use of several culture media (PVK, NBRIP, PSM and Bunt&Rovira) with added dyes used as pH markers (bromphenol blue and bromcresol purple). The used bacteria were three strains of Bacillus amyloliquefaciens (BIR, BW, OS15, OS17), four strains of Bacillus subtilis (BPA, ICPA, ATCC6633, 10), two strains of Bacillus spp. (B3, B4), one strain of Kluyvera ascorbata(35) and one unindentified strain isolated from soil contaminated with swines manure. After 1 to 7 days of incubation, the halos formed around the colonies showed the bacteria capacity to degrade the compounds of interest. Depending on the screening assay it was determined the eventual mechanisms involved, such as organic acids production, phytase or phosphatases synthesis, etc. Bacteria which had the capacity to solublize Pin based compounds were B3, B4, OS15, BPA, BIR, BW, OS17, ATCC6633, 35 and 10. The ones which were able to degrade phytate (Porg) were BIR, BPA, ICPC, OS15, OS17, BW, 10 and 35. The strains that solublized ZnO were BPA, B4, ATCC 6633, 32 and 35. The isolation sources of bacteria were mainly different types of soil, fact that strengthens the teory that soil bacteria are able to secret metabolites which could be involved in bioremediation and crop protection because they are already adapted to soil contaminants from environment.
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