Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XX
Written by Svetlana COLCERU-MIHUL, Sultana NITA, Alice GRIGORE, Corina BUBUEANU, Elena DRAGHICI, Emanuel VAMANU, Domnica RUGHINIS
It is known that oxidative stress and inflammation play an important role in the onset of arterial disorders, very common in the elderly. Cynara scolymus L. is one of the best recommended species for prevention and control of diseases associated with aging processes, mostly due to its high polyphenol content - luteolin, luteolin-7-glucoside, caffeic acid, chlorogenic acid. The aim of the study was to obtain some selective fractions from Cynara scolymus L. leaves with various contents of caffeic, chlorogenic and rosmarinic acids, cynarin, luteolin-7-glucoside, apigenin-7-glucoside and rutin determined by HPLC and to establish the relationship between concentration and antioxidant activity. Eight selective fractions obtained by two distinct methods containing 0-0122% caffeic acid, 0-0.443% rosmarinic acid, 0.007-1.504% chlorogenic acid, 0-0.097% cynarin, 0.054-1.6662% luteolin-7-glucoside, 0.009-1.366% apigenin-7-glucoside and 0-0.396% rutin exhibited antioxidant activity at 0.001, 0.01, 0.1 and 1% dilution, varying from 0.27 to 87.77%. More precisely, selective fraction C6 containing 16.662% luteolin-7-glucoside and C8 selective fraction containing 5.568% luteolin-7-glucoside and 1.504% chlorogenic acid exhibited 87.77%, respectively 84.44% antioxidant activity at 1% dilution and 85.27%, respectively 69.44%. antioxidant activity at 0,1% dilution. The reference substance luteolin-7-glucoside showed 87.16% antioxidant activity at 1% concentration and 85.56% at 0.1% concentration. All selective fractions exhibited antioxidant activity and the action was correlated with their active substances concentration.
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