Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXV, Issue 2
Written by Cristina Valentina ALBU (PROCA), Elias EZZAAF, Diana GROPOȘILĂ-CONSTANTINESCU, Camelia DIGUȚĂ, Gabriela POPA, Florentina MATEI, Călina Petruța CORNEA
The main goal of this study was to optimise the laccase production on laboratory scale, under submerged cultivation, in synthetic media. For this purpose, two Trichoderma spp. strains were isolated from different soil and compost samples. These isolates were then inoculated onto culture media under different pH conditions (4, 5, 6, 7 and 8). To induce laccase production, the culture media were supplemented with two different inductors such as guaiacol 0.03% and 2 - 2.5mM CuSO4. In the first 48 hours of submerged fungal culture, the media with initial pH values in the neutral-basic range (7 and 8), recorded drastically low of pH (between 4 and 5), which suggests an acidification of the culture medium. After 14 days from the experiment initiation, Trichoderma TdMI2 isolate grown on media supplemented with guaiacol, at an initial pH value of 8, had a maximum production of fungal biomass and recorded a slight increase in enzymatic activity (from 0.5 to 0 , 6 U / ml). Between the two isolates of Trichoderma spp - TdCP and TdMI2 - cultured on media supplemented with 2.5 mM CuSO4 as inducer for laccase, only TdMI2 had an enzymatic activity of 0.62 U / ml after 3 days of culture.
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