Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXVI, Issue 1
Written by Nazar AL GHASHEEM, Florin STĂNICĂ, Adrian George PETICILĂ, Oana VENAT, Ana BUTCARU, Imad Al-SUWAID, Basim K. HASAN, Ihasn J. AZEB, Kawther ALGHASHEEM
In vitro three peach cultivars (Florin, Filip and Mimi) included in this experiment, two explants (shoots-tip and nodes) were taken at 0.5-1cm length. The explants were cultivated on 3 medium MS, B5 and QL adding of 30 g/l sucrose and 7 g/l with tested different concentrations of plant hormones BAP and NAA in 12 variants. The aim of this study was to test the effect of culture medium and growth hormones on some peach cultivars. Cultures media MS and QL showed the highest mean number of shoots at 5 mg/l BAP in (MS) medium (Florin 4.20 shoots/explant, Filip 3.60 shoots/explant and Mimi 4.00 shoots/explant respectively), and (QL) medium (Florin 4.40 shoots/explant, Filip 2.40 shoots/explant and Mimi 4.00 shoots/explant) respectively, while the (B5) medium gave the lowest value. Moreover, we were found that there is a relationship between BAP and NAA, since the addition of both hormones has led to the emergence of callus tissue, while adding just BAP to all media in the experiment gave shoots
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