Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXVII, Issue 1
Written by Ketwalee SIWAPITHAKPONG, Sukanya INTHAISONG, Witsarut CHUEAKHUNTHOD, Piyangkoon JAUKWON, Piyada Alisha TANTASAWAT
This study aimed to quantify the recurrent parent genome (RPG) recovery of the new mungbean introgression lines using SSR and EST-SSR markers, and selection of backcross progenies of the crosses between a resistant line (SUPER5) and two recurrent parents (KING and H3). A total of 160 SSR and EST-SSR markers covering 11 mungbean chromosomes were applied for parental polymorphism identification. Among these, 6 polymorphic markers were linked to domestication related traits located on linkage groups (LGs) 1, 3, 7 and 10. Twenty-seven (16.9%) and 23 (14.4%) SSR and EST-SSR markers were found to be distinct and clearly polymorphic between KING and SUPER5, and H3 and SUPER5, respectively. These polymorphic markers were utilized to analyse the RPG recovery in BC1F1 and BC2F1 progenies. The BC2F1 selected progenies with high RPG recovery ranging from 87.2-94.7% and 92.1-97.8% in KING and H3 populations, respectively, were further used to produce BC3F1 seeds. These results suggest that SSR and EST-SSR markers can be used in marker-assisted selection (MAS) for background selection to accelerate backcrossing.
[Read full article] [Citation]
