Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXVIII, Issue 1
Written by Mariana FERDES, Mirela Nicoleta DINCĂ, Mariana IONESCU, Elena Mădălina ȘTEFAN, Alina Daiana IONESCU, Mariana Gabriela MUNTEANU
Five species of edible basidiomycetes - Pleurotus ostreatus, Flammulina filiformis, Ganoderma lucidum, Lentinula edodes, and Polyporus squamosus - were isolated and cultivated as submerged mycelium for the biosynthesis of laccase enzymes and biomass production. The selected mushrooms were grown on different agar media to determine the average growth rate, tested on the Potato Dextrose Agar medium supplemented with guaiacol to determine the laccase enzyme index, and then grown submerged in a rotary incubator, in Erlenmeyer flasks. The development of these species was studied in a medium with sugarcane molasses and mineral salts. The dry biomass of the culture media was determined, and the laccase activity was analysed in the filtrate.
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