Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXVIII, Issue 1
Written by Roxana Mădălina STOICA, Mișu MOSCOVICI, Cristina BÂZDOACĂ, Elena Simina LAKATOS, Lucian Ionel CIOCA
Biosurfactants are surface-active compounds synthesized by microorganisms as secondary metabolites with important applications in medicine, cosmetics, food, oil, agriculture, and the pharmaceutical industries. In the present study, the Pseudomonas fluorescens ICCF 392 strain was screened to determine its ability to produce extracellular biosurfactants. The strain was cultivated on M44 liquid medium (5% (v/v) glycerol as a carbon source) and also, on M44 modified medium (by replacing the glycerol as a carbon source with 5% (v/v) waste cooking oil). The supernatants obtained at the end of the bioprocesses were evaluated, to confirm the ability of the strain in biosurfactant production, using the drop collaps method, oil spreading technique, and emulsification activity determination (E24). The best results were obtained in the case of the M44 liquid medium. The partially purified bioactive compounds were analyzed by the TLC method, which indicated the nature of the biosurfactants produced as rhamnolipids. Therefore, our results showed that the Pseudomonas fluorescens ICCF 392 strain was efficient in biosurfactant production, using glycerol or waste cooking oil as carbon sources in the biosynthesis process.
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