Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXVIII, Issue 2
Written by Asawir Esamaldeen Ebrahim MOHAMED, Oana-Alina BOIU-SICUIA, Călina Petruța CORNEA
This study focuses on Penicillium and similar other mold contaminants found in poultry feed sourced from Sudan. Such fungi were found in sorghum kernels, shelled peanuts, wheat bran and peanut meal from seven Sudanese regions. These fungi were isolated and characterized based on their microscopic features and colony morphology on Dicloran Rose Bengal Chloramphenicol Agar (DRBC), Potato Dextrose Agar (PDA), and Pentachloronitrobenzene - Rose Bengal - Yeast extract - Sucrose Agar (PRYES). To evaluate their mycotoxigenic potential, chromatographic and genetic approaches were used. Fungal extracts were evaluated for mycotoxins content by Thin-Layer Chromatography (TLC). Structural and regulatory genes such as nor-1, ver-1, omt-A, aflR, ota.nps and patN, involved in mycotoxins production were analyzed by classical PCR technology. The strains were also identified by sequencing the ITS1 – 5,8S – ITS2 region. To inhibit the growth of Penicillium isolate certain biocontrol agents were used, revealing good antifungal potential. These findings provide valuable insights into the nature of Penicillium contaminants in Sudanese poultry feed, providing fundamental knowledge for further research on managing mycotoxigenic contaminants and feed safety.
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