Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXIX, Issue 1
Written by Adina Floricica IANCU, Mădălina MILITARU, Sina COSMULESCU
The aim of this study was to construct a bacterial artificial chromosome (BAC) library to confirm the presence and structure of the Rvi2 gene, which provides resistance to apple scab caused by Venturia inaequalis, in several apple cultivars developed in Romania. This approach is intended to aid in detailed genetic analysis to improve apple breeding programs. The clones were sequenced and the results were compared with the sequence of the Rvi2 gene registered in the "National Center for Biotechnology Information" database (locus "AY626824"). BLAST analysis confirmed the presence of the Rvi2 gene in all 20 varieties: 'Alex', 'Bistrițean', 'Cezar', 'Ciprian', 'Dany', 'Discoprim', 'Delicios de Voinesti', 'Estival', 'Ionaprim', 'Luca', 'Pomona', 'Redix', 'Remar',', 'Romus 3', 'Romus 4', 'Romus 5', 'Salva', 'Starkprim’, 'Voinea’ and 'Voincel’. Query identity ranged from 94.6% to 100% for the forward primer and from 95.39% to 100% for the reverse primer, with query coverage ranging from 85% to 92% for the forward primer and 80% to 93% for the reverse primer. The results confirm the presence of the Rvi2 gene, demonstrating high sequence identity and coverage.
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