Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXIX, Issue 1
Written by Behnaz RAHMANIFAR, Oana-Alina BOIU-SICUIA, Oana LIVADARIU, Niculina IONESCU, Călina Petruța CORNEA
Sage (Salvia sp.) is a key medicinal plant from the Lamiaceae family. Recently, the mass production of hairy roots has become important for producing plant secondary metabolites, offering fast growth and stable bioactive compound production, making them ideal for transgenic research. In this study, the optimization of hairy root induction conditions by Agrobacterium rhizogenes in Salvia officinalis, has been evaluated. For this purpose, the effect of 3 different times of bacterial co-culture (24, 48, 72 h) with explants (whole seedling, leaf, stem and petiole) was investigated. The 48h co-culturing interval was considered the most suitable time. The highest rate of success in hairy root production (88.4 %) belonged to the leaf explants. In order to evaluate the stability and quantity of hairy roots, liquid 0.5X MS medium with 3 treatments of IBA (0, 0.5, 1 mg/L) were used. The highest percentage of growth was observed in liquid 0.5X MS and at the hormone level of 1 mg/L. Transgenic hairy roots produced were confirmed by PCR.
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