Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXIX, Issue 1
Written by Elena BIRZA, Nicoleta RUSU, Cătălina VOAIDEȘ, Narcisa Elena BĂBEANU
This study aimed to evaluate the antioxidant activity and mineral content of medlar fruit (Mespilus germanica L.) extracts, using different solvents to identify the most effective extraction method. Extracts were prepared using acetone, ethanol, water, and chloroform. Polyphenol content was analyzed using HPLC, minerals were determined using inductively coupled plasma mass spectrometry (ICP-MS), and antioxidant activity was assessed using chemiluminescence assays to determine IC50 values. The acetonic extract (MP1) had the highest polyphenol content and strong antioxidant activity (AA% of 64%). The aqueous extract (MP2) was richest in minerals. The ethanolic extract (MP3) balanced polyphenol content and antioxidant activity (AA% of 51%). The chloroform extract (MP4) showed a pro-oxidant effect (AA% of -151%). Acetone is the best solvent for extracting polyphenols, while water is ideal for mineral extraction. The ethanolic extract provides a balanced option. Chloroform extracts should be used with caution due to potential pro-oxidant effects. These findings help optimize extraction methods for health benefits, with potential applications in food and pharmaceuticals.
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