Published in Scientific Bulletin. Series F. Biotechnologies, Vol. XXIX, Issue 2
Written by Umesh MADAR, Rajendra PUJAR, Devarajan THANGADURAI, Jeyabalan SANGEETHA, Pavitra CHIPPALAKATTI, Smita SHINDE
Calamus nagbettai R.R.Fernald and Dey are spiny climbing palms belonging to the family Arecaceae which are mainly used for making furniture products and handcrafts. DNA markers have been employed to estimate the level of genetic diversity and distinguish the distribution patterns of genetic deviation within the populations. Studies on the genetic dissimilarity in twenty-six populations of Calamus nagbettai have been assisted by the development of molecular markers. Seven Random Amplified Polymorphic DNA (RAPD) markers were utilised to evaluate genetic diversity and genetic relationships between twenty-six accessions of Calamus nagbettai. The primers OPA-03 produced the maximum number of amplicons (260 amplicons), whereas OPA- 06 produced the least number of loci (75 amplicons). Genetic similarity coefficients among 26 wild populations of C. nagbettai ranged from 0.9 to 1.00 which was calculated using Jaccard’s similarity coefficient. The dendrogram was constructed for RAPD data for all the 26 accessions of Calamus nagbettai which formed 2 major clusters A and B. The study concludes that RAPD is a potential molecular marker to evaluate the genetic diversity of C. nagbettai species.
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